EVIDENCE THAT TOTAL SUBSTITUTION OF ADENINE WITH 7-DEAZAADENINE IN THE HDV ANTIGENOMIC RIBOZYME CHANGES THE KINETICS OF RNA FOLDING

Authors
WIECZOREK A DINTERGOTTLIEB C GOTTLIEB PA
Citation
A. Wieczorek et al., EVIDENCE THAT TOTAL SUBSTITUTION OF ADENINE WITH 7-DEAZAADENINE IN THE HDV ANTIGENOMIC RIBOZYME CHANGES THE KINETICS OF RNA FOLDING, Bioorganic & medicinal chemistry letters, 4(8), 1994, pp. 987-994
Citations number
18
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
4
Issue
8
Year of publication
1994
Pages
987 - 994
Database
ISI
SICI code
0960-894X(1994)4:8<987:ETTSOA>2.0.ZU;2-K
Abstract
We incorporated 7-deazaadenine residues in place of adenine in an in v itro transcribed ribozyme derived from the Hepatitis delta virus. The rate of self-cleavage was unchanged, but the modified RNA cleavage was disrupted by any addition of formamide. Similarly, an increase in mag nesium ion was necessary to support the self-cleavage reaction in the modified RNA.