THE PREPARATION OF ENZYME-LABELED OLIGONUCLEOTIDES BY REDUCTIVE AMINATION

Oligonucleotides containing an aldehyde group at the 3'-terminus have been prepared using a derivatized solid phase. This solid phase contai ned a short peptide that included a lysine residue substituted at the N-epsilon-amino group with carboxybenzaldehyde. The oligonucleotide wa s coupled to alkaline phosphatase under reductive amination conditions to produce a conjugate with unaltered hybridization properties.