EFFECTS OF 5-METHYL SUBSTITUTION IN 2'-O-METHYLOLIGO(PYRIMIDINE)NUCLEOTIDES ON TRIPLE-HELIX FORMATION

Authors
SHIMIZU M KOIZUMI T INOUE H OHTSUKA E
Citation
M. Shimizu et al., EFFECTS OF 5-METHYL SUBSTITUTION IN 2'-O-METHYLOLIGO(PYRIMIDINE)NUCLEOTIDES ON TRIPLE-HELIX FORMATION, Bioorganic & medicinal chemistry letters, 4(8), 1994, pp. 1029-1032
Citations number
19
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
4
Issue
8
Year of publication
1994
Pages
1029 - 1032
Database
ISI
SICI code
0960-894X(1994)4:8<1029:EO5SI2>2.0.ZU;2-I
Abstract
A 2'-O-methyloligo(pyrimidine)nucleotide containing a 5-methyl-2'-O-me thyluridine (I) forms a more stable triple-helix with double-stranded DNA than the 5-unmodified parent probe, whereas 5-methyl-2'-O-methylcy tidine (II) destabilizes the tripler. It is also shown that at low sal t concentrations, the triplexes with 2'-O-methyl RNAs are more stable than the corresponding DNA-triplex.