ISOLATION OF MONOCHROMOSOMAL HYBRIDS FOR MOUSE CHROMOSOME-3, CHROMOSOME-6, CHROMOSOME-10, CHROMOSOME-12, CHROMOSOME-14, AND CHROMOSOME-18

Authors
SABILE A PORAS I CHERIF D GOODFELLOW P AVNER P
Citation
A. Sabile et al., ISOLATION OF MONOCHROMOSOMAL HYBRIDS FOR MOUSE CHROMOSOME-3, CHROMOSOME-6, CHROMOSOME-10, CHROMOSOME-12, CHROMOSOME-14, AND CHROMOSOME-18, Mammalian genome, 8(2), 1997, pp. 81-85
Citations number
40
Categorie Soggetti
Biology,"Genetics & Heredity","Biothechnology & Applied Migrobiology
Journal title
Mammalian genome → ACNP
ISSN journal
09388990
Volume
8
Issue
2
Year of publication
1997
Pages
81 - 85
Database
ISI
SICI code
0938-8990(1997)8:2<81:IOMHFM>2.0.ZU;2-Y
Abstract
Mouse/human somatic cell hybrids constitute a valuable resource for bo th genetic and physical mapping. In this report, we describe the produ ction and characterization of a series of six monochromosomal hybrids generated by fusion of murine microcells with intact human recipient c ells. The presence of each mouse chromosome was characterized by PCR a nalysis and the integrity of the mouse chromosome retained in the hybr ids confirmed by fluorescence in situ hybridization (FISH) analysis.