FLOW-INJECTION IMMUNOSENSOR FOR TRIAZINE HERBICIDES USING EU(III) CHELATE LABEL FLUORESCENCE DETECTION

An immunochemical flow-injection system for the determination of triaz ine herbicides based on principles of immunoaffinity chromatography wa s developed. Triazine herbicide derivatives immobilized on oxirane acr ylic beads serve as the affinity column. They are saturated with fluor escently labelled monoclonal anti-herbicide antibodies prior to the as say. The label used is the fluorescent Eu(III) chelate W8044-Eu. A fra ction of the fluorescent antibodies is replaced when exposed to analyt e and this fluorescence is detected in a postcolumn mode by means of a special laser-based fluorimeter. With the reusable affinity column a detection limit of 1 mu g 1(-1) for the herbicide atrazine was obtaine d.