SYNTHESIS OF FUNCTIONAL GABA(A) RECEPTORS IN STABLE INSECT-CELL LINES

We have synthesised the beta 1-subunit of the bovine GABA, receptor in stable, continuous insect (Spodoptera frugiperda) cell lines. A cDNA was integrated randomly into the insect cell genome under control of a baculovirus immediate early (IE-1) gene promoter. Transformed cells w ere obtained by co-transfection of the insect cells with pIEK1.GR beta 1, encoding the beta 1 subunit cDNA, and pIEK1.neo, encoding the neom ycin resistance gene. G-418-resistant clones were selected and expande d into continuous cell lines synthesising functional, GABA-gated, homo -oligomeric chloride channels. These cell lines had significant advant ages over the transient baculovirus expression system for the characte risation of receptors using electrophysiological recording techniques.