RPII15 CODES FOR THE M(R)-15,000 SUBUNIT-9 OF DROSOPHILA-MELANOGASTERRNA-POLYMERASE-II

The RPII15 gene product of Drosophila melanogaster, which has recently been identified by sequence comparison, possesses a high similarity t o subunit 9 of yeast RNA polymerase II. Using the polymerase chain rea ction the coding region of RPII15 was isolated from genomic DNA of adu lt flies. Sequence analysis shows four amino acid substitutions in com parison to the previously reported sequence. Antisera were generated a gainst bacterially expressed RPII15 and were used for immunoblotting e xperiments with RNA polymerase II of Drosophila melanogaster. This ana lysis identified the M(r) 15,000 subunit 9 as gene product of RPII15.