SYNTHESIS AND CONNECTIVITY ASSIGNMENT (BY 2D-NMR) OF A NUCLEOSIDE-DIPEPTIDE - YL]AMINO]ETHYL]AMINO]-3-OXOPROPYL]-2'-DEOXYURIDINE

The synthesis of VI, a 2'-deoxyuridine derivative with a His-His dipep tide covalently attached at the pyrimidine C-5 position through a link er arm, is reported. The title compound is designed to serve as a buil ding block for catalytic nucleic acids, or synthetic ribozymes, and wa s prepared in five steps from 2-amino-ethyl)amino]-3-oxopropyl]-2'-deo xyuridine. The complete connectivity assignment of VI and extensive NM R assignment of the intermediates II-V by the use of 1- and 2-DNMR are reported.