ORIGIN OF MULTIPLE BANDS OF PROTEINS ON SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS - INTERMOLECULAR DISULFIDE CROSS-LINKING DUE TO THE PRESENCE OF OXIDIZING COMPONENTS IN THE REDUCING AGENT

Authors
KUMAR TKS PRASAD VVH PANDIT MW
Citation
Tks. Kumar et al., ORIGIN OF MULTIPLE BANDS OF PROTEINS ON SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS - INTERMOLECULAR DISULFIDE CROSS-LINKING DUE TO THE PRESENCE OF OXIDIZING COMPONENTS IN THE REDUCING AGENT, Journal of biochemical and biophysical methods, 28(3), 1994, pp. 243-247
Citations number
9
Categorie Soggetti
Biology,Biophysics,"Biochemical Research Methods
Journal title
Journal of biochemical and biophysical methods → ACNP
ISSN journal
0165022X
Volume
28
Issue
3
Year of publication
1994
Pages
243 - 247
Database
ISI
SICI code
0165-022X(1994)28:3<243:OOMBOP>2.0.ZU;2-0
Abstract
The presence of oxidized products in the reducing agent used in sodium dodecyl sulphate-polyacrylamide gel electrophoresis is shown to yield multiple bands from otherwise homogeneous RNase A. The role of oxidiz ed products in generating multiple bands is elucidated by using varyin g proportions of oxidized and reduced glutathione in a mixture as a re ducing agent.