ELECTROSPRAY MASS-SPECTROMETRIC CHARACTERIZATION OF THE COMPONENTS OFPROTEIN MIXTURES AND ITS APPLICATION TO MEMBERS OF THE CHEMOKINE FAMILY OF INTERLEUKINS

Authors
KAY I HAFIZI S BARR RM MALLET AI GREAVES MW
Citation
I. Kay et al., ELECTROSPRAY MASS-SPECTROMETRIC CHARACTERIZATION OF THE COMPONENTS OFPROTEIN MIXTURES AND ITS APPLICATION TO MEMBERS OF THE CHEMOKINE FAMILY OF INTERLEUKINS, Biological mass spectrometry, 23(5), 1994, pp. 267-271
Citations number
12
Categorie Soggetti
Spectroscopy,Biophysics
Journal title
Biological mass spectrometry → ACNP
ISSN journal
10529306
Volume
23
Issue
5
Year of publication
1994
Pages
267 - 271
Database
ISI
SICI code
1052-9306(1994)23:5<267:EMCOTC>2.0.ZU;2-V
Abstract
Electrospray mass spectrometry has been employed to detect and charact erize the members of a closely related family of immunologically activ e proteins (chemokines) produced in cell culture by stimulated fibrobl asts. The reliability of the method to produce precise data concerning the relative proportions of proteins present in mixtures was investig ated in a model system and found to be satisfactory in a range of 10:1 in concentration.