ISOLATION BY HPLC OF THE POSITIONAL ISOMERS OF (N)-DI-O-ALPHA-D-GALACTOPYRANOSYLCYCLOMALTOHEXAOSE (N=1-6) AND DETERMINATION OF THEIR STRUCTURES BY ENZYMATIC DEGRADATION

The six positional isomers of (n)-di-O-alpha-D-galactopyranosylcycloma ltohexaose (alpha-cyclodextrin, alpha CD, n = 1-6), which were produce d from alpha CD and melibiose by transgalactosylation with coffee bean alpha-galactosidase, were isolated by HPLC on a reversed-phase column and a graphitized carbon column. The structures of those isomers were elucidated by LC-MS analyses of enzymatic degradation products with c yclomaltodextrin glucanotransferase and alpha-galactosidase. (C) 1997 Elsevier Science Ltd.