DISTINGUISHING ISOLATES OF PERONOSPORA-TABACINA FROM GEOGRAPHIC REGIONS UTILIZING TOBACCO LEAF-DISKS AND FLUORESCENCE MICROSCOPY

Authors
WIGLESWORTH MD NESMITH WC SIEGEL MR BONDE MR MAIN CE
Citation
Md. Wiglesworth et al., DISTINGUISHING ISOLATES OF PERONOSPORA-TABACINA FROM GEOGRAPHIC REGIONS UTILIZING TOBACCO LEAF-DISKS AND FLUORESCENCE MICROSCOPY, Plant disease, 78(5), 1994, pp. 456-460
Citations number
21
Categorie Soggetti
Plant Sciences
Journal title
Plant disease → ACNP
ISSN journal
01912917
Volume
78
Issue
5
Year of publication
1994
Pages
456 - 460
Database
ISI
SICI code
0191-2917(1994)78:5<456:DIOPFG>2.0.ZU;2-L
Abstract
A laboratory bioassay to distinguish isolates of Peronospora tabacina D.B. Adam was developed utilizing 7-mm acetone-dipped disks cut from t obacco leaf panels from several cultivars of Nicotiana tabacum, sporan giospores of P. tabacina, a germination indicator (Calcofluor), and fl uorescence microscopy. Sporangiospore germination percentage of each i solate X cultivar pair was determined. Mean germination of sporangiosp ores differed between domestic and international collection locations (except between the two Mexican isolates). These results suggest the p resence of different pathotypes of P. tabacina.