Resistance to pentachloronitrobenzene (PCNB) was not detected among 11
2 isolates of Sclerotium rolfsii collected from commercial peanut fiel
ds in Oklahoma and assayed for inhibition of radial growth in vitro. V
alues of PCNB concentration that provided 50% inhibition of growth (EC
50) were normally distributed about a mean of 2.86 +/- 1.45 mug/ml. An
isolate of S. rolfsii from apple, collected where PCNB had never been
used, had a comparable EC50 of 3.29 +/- 0.18 mug/ml. The recommended
PCNB use pattern, one or two applications at 0.56 kg/ha in a wide (30-
cm) band over the row, gave variable control of southern blight (23-90
%) and peanut yield increases (102-915 kg/ha) in five field trials. In
field trials comparing different combinations of PCNB rates and band
widths, half rates (2.8 kg/ha) applied in a wide band resulted in no d
isease control in one trial where full rates (5.6 kg/ha) in a wide ban
d and half rates in a narrow (10-cm) band were effective. None of the
PCNB treatments were effective in a second trial. Increasing the conce
ntration of PCNB at the plant crown with full rates in a narrow band d
id not improve disease control compared to full rates in a wide band.
One (1.12 kg/ha) or two (0.84 kg/ha) banded sprays with flutolanil or
three foliar sprays of tebuconazole (0.14-0.16 kg/ha) provided more co
nsistent disease control than did PCNB. However, yield increases from
the experimental fungicides were not always greater than those from PC
NB nor were they as large as those reported in areas where yields are
higher and both southern blight and Rhizoctonia limb rot are problems.
EC50 values for isolates of S. rolfsii were determined for flutolanil
(0.08 +/- 0.04 mug/ml) and tebuconazole (0.06 +/- 0.02 mug/ml) for re
ferences in resistance monitoring if these fungicides are registered i
n the future.