VECTORS FOR CU2-INDUCIBLE PRODUCTION OF GLUTATHIONE S-TRANSFERASE-FUSION PROTEINS FOR SINGLE-STEP PURIFICATION FROM YEAST()

Authors
WARD AC CASTELLI LA MACREADIE IG AZAD AA
Citation
Ac. Ward et al., VECTORS FOR CU2-INDUCIBLE PRODUCTION OF GLUTATHIONE S-TRANSFERASE-FUSION PROTEINS FOR SINGLE-STEP PURIFICATION FROM YEAST(), Yeast, 10(4), 1994, pp. 441-449
Citations number
16
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology",Biology
Journal title
Yeast → ACNP
ISSN journal
0749503X
Volume
10
Issue
4
Year of publication
1994
Pages
441 - 449
Database
ISI
SICI code
0749-503X(1994)10:4<441:VFCPOG>2.0.ZU;2-I
Abstract
We describe six new yeast episomal vectors which encode glutathione S- transferase (GST) affinity tags. These allow for the production of GST -fusion proteins in Saccharomyces cerevisiae under the control of the CUP1 promoter. Affinity chromatography with glutathione-Sepharose perm its convenient purification of the fusion protein from a yeast lysate. The presence of a protease cleavage site facilitates subsequent remov al of the GST tag. The expression and single-step purification of both GST and a functional GST-metallothionein fusion from yeast are shown as an example of the application of these vectors.