M. Ziman et Di. Johnson, GENETIC-EVIDENCE FOR A FUNCTIONAL INTERACTION BETWEEN SACCHAROMYCES-CEREVISIAE CDC24 AND CDC42, Yeast, 10(4), 1994, pp. 463-474
Cdc24p and Cdc42p are involved in the control of cell polarity during
the Saccharomyces cerevisiae cell cycle. Cdc42p is a member of the Ras
superfamily of GTPases and Cdc24p displays limited amino-acid sequenc
e similarity with the Dbl proto-oncoprotein, which acts to stimulate g
uanine-nucleotide exchange on human Cdc42p. We have performed several
genetic experiments to test whether Cdc24p and Cdc42p interact within
the cell. First, overexpression of Cdc24p suppressed the dominant-nega
tive cdc42(D118A) allele. Second, overexpression of wild-type CDC24 an
d CDC42 genes together was a lethal event resulting in a morphological
phenotype of large, round, unbudded cells, indicating a loss of cell
polarity. Third, a cdc24(ts) cdc42(ts) double mutant exhibited a synth
etic-lethal phenotype at the semi-permissive temperature of 30 degrees
C. These data suggest that Cdc24p and Cdc42p interact within the cell
and that Cdc24p may be involved in the regulation of Cdc42p activity.