SEQUENCE, MAPPING AND DISRUPTION OF CCC1, A GENE THAT CROSS-COMPLEMENTS THE CA2-SENSITIVE PHENOTYPE OF CSG1 MUTANTS()

We have isolated, sequenced, mapped and disrupted a novel gene, CCC1, from Saccharomyces cerevisiae. This gene displays non-allelic compleme ntation of the Ca2+-sensitive phenotype conferred by the csgl mutation . The ability of this gene, in two copies per cell, to reverse the csg l defect suggests it may have a role in regulating Ca2+ homeostasis. T he sequence of CCC1 indicates that it encodes a 322 amino acid, membra ne-associated protein. The CCC1 gene is located on the right arm of ch romosome XII. The sequence has been deposited in the GenBank data libr ary under Accession Number L24112.