T. Badzian et Jj. Rybczynski, CYTOKININ CONTROL OF SHOOT REGENERATION IN ROOT SEGMENT CULTURE OF LOTUS-CORNICULATUS SEEDLING, Acta Physiologiae Plantarum, 16(1), 1994, pp. 61-67
An investigation was carried out to describe the infuence of four cyto
kinins: kinetin, zeatin, 2iP and BA on shoot formation in 5 mm long no
n-meristematic root segments of Lotus corniculatus L. cv. Noreen seedl
ing. Two step culture protocol was used: the first four weeks of cultu
re comprised bud induction in the presence of cytokinins and the secon
d three weeks opportunity to develop from bud to shoot on basal MS-med
ium without cytokinins. When describing the cytokinin influence on sho
ot formation the following criteria were taken under consideration; nu
mber of regenerated adventitious shoots per explant, good development
of adventitious shoot per explant, good development of bud , lack of s
hoot deformation or vitrification and the smallest number of dying exp
lants in replication. The most effective cytokinin appeared to be BA a
t low concentrations : 0.1 and 0.25 mg/l. Rooting processes of explant
s were inhibited by high cytokinin concentration.