CYTOKININ CONTROL OF SHOOT REGENERATION IN ROOT SEGMENT CULTURE OF LOTUS-CORNICULATUS SEEDLING

An investigation was carried out to describe the infuence of four cyto kinins: kinetin, zeatin, 2iP and BA on shoot formation in 5 mm long no n-meristematic root segments of Lotus corniculatus L. cv. Noreen seedl ing. Two step culture protocol was used: the first four weeks of cultu re comprised bud induction in the presence of cytokinins and the secon d three weeks opportunity to develop from bud to shoot on basal MS-med ium without cytokinins. When describing the cytokinin influence on sho ot formation the following criteria were taken under consideration; nu mber of regenerated adventitious shoots per explant, good development of adventitious shoot per explant, good development of bud , lack of s hoot deformation or vitrification and the smallest number of dying exp lants in replication. The most effective cytokinin appeared to be BA a t low concentrations : 0.1 and 0.25 mg/l. Rooting processes of explant s were inhibited by high cytokinin concentration.