CHOICE OF STATIONARY AND MOBILE PHASES FO R SEPARATION OF MIXED TRIGLYCERIDES BY LIQUID-PHASE CHROMATOGRAPHY

A systematic study of retention of homogenous saturated and unsaturate d triglycerides has been made on different stationary phases (octadecy l, phenyl, mixed C18-CN bonded silicas) with various non-aqueous binar y and ternary mobile phases. It allowed to determine optimal and simpl e conditions of analysis of mixed triglycerides in fats or oils. The u se of combined optimization criteria to judge the quality of an entire chromatogram leads to propose the use of one column with polymeric be havior, with a binary and isocratic mobile phase (MeCN/CH2Cl2). The tr iglyceride composition being complex, the entire identification requir ed fuller studies of the oils and fats by mass spectrometry/gas chroma tography and mass spectrometry/liquid chromatography.