Sterols were identified and quantified in six marine microalgal strain
s identified as belonging to the prymnesiophyte genus Isochrysis; seve
ral of these strains are in wide use in the commerical mariculture ind
ustry. One strain contained only cholesterol, and another contained a
complex mixture of Delta(5)-sterols and dihydroxysterols. Two strains
contained brassicasterol and two others contained epibrassicasterol as
the principal sterol. The strains containing brassicasterol or epibra
ssicasterol also contained long chain alkenones characteristic of some
members of the Prymnesiophyceae; whereas, those without brassicastero
l or epibrassicasterol contained no long chain alkenones. These qualit
ative biochemical differences appear to have taxonomic significance an
d may be important in the value of these algae as live feeds for reari
ng marine invertebrates.