ELUTRIATION OF T-LYMPHOBLASTIC CELLS AND CELL-CYCLE ANALYSIS OF FRACTIONS

Elutriation of human lymphoblastic cells has to be performed at a flow rate of 13 ml/min at a temperature of 10 degrees C to retain a high c ell viability. Cell cycle analysis of elutriated cells on the basis of their DNA contents is inaccurate. The combined detection of BrdUrd in corporated into the DNA and analysis of the DNA content offers an alte rnative. After elutriation, fractions of cells with increased percenta ges of either G1 or S phase cells can be selected on the basis of the median cell size as established by coulter counter channelyzer.