ESTABLISHMENT AND CHARACTERIZATION OF 2 NEW HUMAN RENAL-CANCER CELL-LINES (DNT-11 AND DNT-63) DERIVED FROM METASTASES

Two new human cell lines (DNT-11 and DNT-63) derived from renal cell c arcinoma metastases were established and characterized. They have been passaged up to 50 times in vitro for more than 4 years. Each line has an epithelial morphology. Cell cycle time of DNT-11 and DNT-63 was 60 hours and 48 hours, respectively. Both cell lines expressed HLA class I antigens, but were found to be negative for HLA class II. DNT-11 an d DNT-63 had a hyperdiploid DNA content. They exhibited anchorage inde pendent growth, and the plating efficiency of DNT-11 and DNT-63 was 0. 85% and 1.65%, respectively. These two cell lines may provide a useful tool for studying the biology and immunology of metastatic renal cell carcinoma.