(CAC)(5) DETECTS DNA FINGERPRINTS AND SEQUENCES HOMOLOGOUS TO GENE TRANSCRIPTS IN RICE

In the present report the potential of (CAC)(5) is demonstrated for DN A fingerprinting in rice. Based on the fingerprint data, (CAC)(5) was estimated to differentiate up to 6.3 x 10(8) genotypes of rice. In a s earch for homologous sequences in rice transcripts, four oligonucleoti de probes, namely, (CAC)(5), (GATA)(4), (GACA)(4), and (TG)(10), were used. Among these probes, (CAC)(5) showed hybridization to total rice RNA in a tissue-specific manner; while a band at 1.2 kb was common in both seed and leaf RNA, there were additional prominent bands at 1.9 a nd 2.3 kb in seed and leaf RNA, respectively. When (CAC)(5) was hybrid ized to poly(A)(+) RNA, in addition to a band at 1.2 kb in seed and le af hybridization was observed only in seed to heterogeneously sized RN As.