ISOLATION OF CELLULASE HYPER-PRODUCING STRAINS FROM HYPERPOLYPLOIDS OF TRICHODERMA-REESEI

Swollen conidia derived from Trichoderma reesei QM 6a were treated wit h 0.1% (w/v) colchicine solution in order to form hyperpolyploids. Man y sectors were generated from colonies of the hyperpolyploids after be nlate treatment. Cellulase hyper-producers were selected using Avicel layer solid medium among conidia generated on sectors. In the strain B 111, Avicel, cellulose powder, CMC-Na, and salicin hydrolysing activit ies were 2.2, 1.7, 26.4, and 4.8 times higher, respectively, than thos e of the original strain. It is suggested that hyperpolyploids of this fungus may contribute to the formation of cellulase hyper-producing s trains.