ROLE OF ADENOSINE IN PRESERVATION BY THE 2-LAYER METHOD OF ISCHEMICALLY DAMAGED CANINE PANCREAS

Citation
Y. Kuroda et al., ROLE OF ADENOSINE IN PRESERVATION BY THE 2-LAYER METHOD OF ISCHEMICALLY DAMAGED CANINE PANCREAS, Transplantation, 57(7), 1994, pp. 1017-1020
Citations number
26
Categorie Soggetti
Immunology,Surgery
Journal title
ISSN journal
00411337
Volume
57
Issue
7
Year of publication
1994
Pages
1017 - 1020
Database
ISI
SICI code
0041-1337(1994)57:7<1017:ROAIPB>2.0.ZU;2-P
Abstract
The purpose of this study was to clarify the role of adenosine in pres ervation of ischemically damaged pancreas by the two-layer (Euro-Colli ns solution [EC]I perfluorochemical [PFC]) method using a canine model . Twenty-four-hour preservation of the pancreas graft subjected to 60- min warm ischemia was successful by the two-layer (EC with adenosine/P FC) method (4/5, 80%), but neither simple cold storage in EC (0/5, 0%) , nor EC with adenosine (1/5, 20%), nor the two-layer (EC/PFC) method (0/3, 0%) was successful. Tissue ATP concentrations at the end of pres ervation by the two-layer (EC with adenosine/PFC) method were signific antly higher compared with the two-layer (EC/PFC) method (7.23+/-2.17 vs. 1.56+/-0.40 mu mol/g dry weight, P<0.01). Studies with [2-H-3]aden osine demonstrated that only part of adenosine was converted to inosin e, hypoxanthine, and adenine, whereas the remainder was incorporated i nto adenine nucleotides in the pancreas graft. In addition, hypoxanthi ne, inosine, and adenine did not substitute for adenosine. We conclude that provision of adenosine to ischemically damaged pancreas during p reservation by the two-layer (EC/PFC) method allows ATP synthesis with in the graft via direct phosphorylation of adenosine. Metabolic proces ses vital to repair damaged cells and maintain cellular integrity can be maintained, which makes it possible to preserve ischemically damage d pancreas.