Y. Kuroda et al., ROLE OF ADENOSINE IN PRESERVATION BY THE 2-LAYER METHOD OF ISCHEMICALLY DAMAGED CANINE PANCREAS, Transplantation, 57(7), 1994, pp. 1017-1020
The purpose of this study was to clarify the role of adenosine in pres
ervation of ischemically damaged pancreas by the two-layer (Euro-Colli
ns solution [EC]I perfluorochemical [PFC]) method using a canine model
. Twenty-four-hour preservation of the pancreas graft subjected to 60-
min warm ischemia was successful by the two-layer (EC with adenosine/P
FC) method (4/5, 80%), but neither simple cold storage in EC (0/5, 0%)
, nor EC with adenosine (1/5, 20%), nor the two-layer (EC/PFC) method
(0/3, 0%) was successful. Tissue ATP concentrations at the end of pres
ervation by the two-layer (EC with adenosine/PFC) method were signific
antly higher compared with the two-layer (EC/PFC) method (7.23+/-2.17
vs. 1.56+/-0.40 mu mol/g dry weight, P<0.01). Studies with [2-H-3]aden
osine demonstrated that only part of adenosine was converted to inosin
e, hypoxanthine, and adenine, whereas the remainder was incorporated i
nto adenine nucleotides in the pancreas graft. In addition, hypoxanthi
ne, inosine, and adenine did not substitute for adenosine. We conclude
that provision of adenosine to ischemically damaged pancreas during p
reservation by the two-layer (EC/PFC) method allows ATP synthesis with
in the graft via direct phosphorylation of adenosine. Metabolic proces
ses vital to repair damaged cells and maintain cellular integrity can
be maintained, which makes it possible to preserve ischemically damage
d pancreas.