THE USE OF FISH SCALE EPITHELIA IN NONDESTRUCTIVE METHODS OF KARYOTYPING OREOCHROMIS-NILOTICUS L AND CYPRINUS-CARPIO L FOR EXPERIMENTAL GYNOGENETIC STUDIES
S. Peruzzi et al., THE USE OF FISH SCALE EPITHELIA IN NONDESTRUCTIVE METHODS OF KARYOTYPING OREOCHROMIS-NILOTICUS L AND CYPRINUS-CARPIO L FOR EXPERIMENTAL GYNOGENETIC STUDIES, Caryologia, 47(1), 1994, pp. 39-45
Metaphase figures suitable for karyological investigations in fish wer
e produced by the use of non-invasive and non-destructive methodologie
s. Large numbers of dividing scale epithelial cells were obtained by t
wo methods: first, scale epithelia of Oreochromis niloticus were cultu
red for 22 hours at 28-degrees-C, and second, scale epithelia of Cypri
nus carpio were used without culturing after the addition of a metapha
se inhibitor. These techniques gave typical karyotypes in control carp
and tilapia and confirmed absence of paternal (carp) chromosomes in g
ynogenetic tilapias.