OPTIMIZATION OF FLAME-BASED SULFUR CHEMILUMINESCENCE DETECTION WITH MICROCOLUMN HIGH-PERFORMANCE REVERSED-PHASE LIQUID-CHROMATOGRAPHY

Authors
HOWARD AL THOMAS CLB TAYLOR LT
Citation
Al. Howard et al., OPTIMIZATION OF FLAME-BASED SULFUR CHEMILUMINESCENCE DETECTION WITH MICROCOLUMN HIGH-PERFORMANCE REVERSED-PHASE LIQUID-CHROMATOGRAPHY, Analytical chemistry, 66(9), 1994, pp. 1432-1437
Citations number
10
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
66
Issue
9
Year of publication
1994
Pages
1432 - 1437
Database
ISI
SICI code
0003-2700(1994)66:9<1432:OOFSCD>2.0.ZU;2-C
Abstract
Through a detailed optimization process, response surfaces were obtain ed for micro-HPLC/SCD under various reversed-phase (methanol/water) el ution conditions. Linear dynamic range, correlation coefficient, respo nse factor, and limit of detection were found to vary with mobile-phas e composition. The lowest sensitivity achieved was a detectivity of 60 0 fg of S/s at a mobile-phase composition of 40% methanol/60% water. A pplication of the HPLC/SCD interface to the separation of thiocarbamat es extracted from apples is presented.