A COMPETITIVE ENZYME-IMMUNOASSAY SUBCLASS((R)) FOR THE DETERMINATION OF TOTAL IGG-SUBCLASS LEVELS IN HUMAN SERUM - COMPARISON WITH SINGLE RADIAL IMMUNODIFFUSION

Authors
MARANON F CASANOVAS M BERRENS L OLLES JM DIEGUEZ MA
Citation
F. Maranon et al., A COMPETITIVE ENZYME-IMMUNOASSAY SUBCLASS((R)) FOR THE DETERMINATION OF TOTAL IGG-SUBCLASS LEVELS IN HUMAN SERUM - COMPARISON WITH SINGLE RADIAL IMMUNODIFFUSION, Journal of immunoassay, 15(2), 1994, pp. 147-156
Citations number
9
Categorie Soggetti
Immunology
Journal title
Journal of immunoassay → ACNP
ISSN journal
01971522
Volume
15
Issue
2
Year of publication
1994
Pages
147 - 156
Database
ISI
SICI code
0197-1522(1994)15:2<147:ACESFT>2.0.ZU;2-O
Abstract
A quantitative immunoassay has been developed for the determination of the total IgG subclass levels in human serum. The method is based on an enzyme immunoassay in which IgG subclass proteins IgGx in an unknow n serum sample compete with a known quantity of peroxidase (PO)-labell ed IgGx in fluid phase for subclass-specific monoclonal antibodies coa ted to the solid phase of microtiter wells. Using a series of human bl ood samples an excellent correlation was observed with the IgG-subclas s levels determined by single radial immunodiffusion.