NATIVE ZONA-PELLUCIDA STRUCTURE IS REQUIRED FOR COMPLETION OF SPERM ACROSOME REACTION IN PORCINE FERTILIZATION

Authors
YOSHIZAWA M NAGAI T YONEZAWA N NAKANO M
Citation
M. Yoshizawa et al., NATIVE ZONA-PELLUCIDA STRUCTURE IS REQUIRED FOR COMPLETION OF SPERM ACROSOME REACTION IN PORCINE FERTILIZATION, Theriogenology, 41(6), 1994, pp. 1307-1313
Citations number
13
Categorie Soggetti
Veterinary Sciences
Journal title
Theriogenology → ACNP
ISSN journal
0093691X
Volume
41
Issue
6
Year of publication
1994
Pages
1307 - 1313
Database
ISI
SICI code
0093-691X(1994)41:6<1307:NZSIRF>2.0.ZU;2-I
Abstract
In fertilization in vitro, the penetration rate of zona-intact porcine oocytes by cryopreserved epididymal spermatozoa was about 100% while that of zona-free oocytes was only 30%. Spermatozoa treated with calci um ionophore A23187 penetrated both and zona-free oocytes at the rate of more than 90%. of spermatozoa with solubilized porcine zonae pelluc idae hardly induced acrosome reaction and did not increase the penetra tion rate. These results suggest that the structure of the zona is nec essary for completion of acrosome reaction.