PROTON NMR-STUDIES ON THE PERMEATION OF TISSUE FRAGMENTS BY DIMETHYL-SULFOXIDE - LIVER AS A MODEL FOR COMPACT TISSUES

Authors
FULLER BJ BUSZA AL
Citation
Bj. Fuller et Al. Busza, PROTON NMR-STUDIES ON THE PERMEATION OF TISSUE FRAGMENTS BY DIMETHYL-SULFOXIDE - LIVER AS A MODEL FOR COMPACT TISSUES, Cryo-letters, 15(2), 1994, pp. 131-134
Citations number
10
Categorie Soggetti
Biology Miscellaneous",Physiology
Journal title
Cryo-letters → ACNP
ISSN journal
01432044
Volume
15
Issue
2
Year of publication
1994
Pages
131 - 134
Database
ISI
SICI code
0143-2044(1994)15:2<131:PNOTPO>2.0.ZU;2-P
Abstract
Data on the permeability of liver tissue to the cryoprotective agent d imethyl sulphoxide (Me2SO) by diffusion have been obtained non-invasiv ely using H-1 nuclear magnetic resonance (NMR) spectroscopy during bot h exposure and washout. Results demonstrate that in compact tissues eq uilibration with tissue water is not complete for up to 2 hrs, while t he kinetics for influx and efflux were similar. These observations are discussed in relation to the development of protocols for cryoprotect ant exposure in tissue cryopreservation.