BNAC1 AND BNAC2 CONSTITUTE AT NEW FAMILY OF HUMAN NEURONAL SODIUM-CHANNELS RELATED TO DEGENERINS AND EPITHELIAL SODIUM-CHANNELS

The recently defined DEG/ENaC superfamily of sodium channels includes subunits of the amiloride-sensitive epithelial sodium channel (ENaC) o f vertebrate colon, lung, kidney, and tongue, a molluscan FMRFamidegat ed channel (FaNaC), and the nematode degenerins, which are suspected m echanosensory channels, We have identified two new members of this sup erfamily (BNaC1 and BNaC2) in a human brain cDNA library, Phylogenetic analysis indicates they are equally divergent from all other members of the DEG/ENaC superfamily and form a new branch or family, Human BNa C1 maps to 17q11.2-12 and hBNaC2 maps to 12q12, Northern blot and mous e brain in situ hybridizations indicate that both genes are coexpresse d in most if not all brain neurons, although their patterns of express ion vary slightly, and are expressed early in embryogenesis and throug hout life. By analogy to the ENaCs and the degenerins, which form hete romultimeric channels, BNaC1 and BNaC2 may be subunits of the same cha nnel.