THE ADE4 GENE OF SCHIZOSACCHAROMYCES-POMBE - CLONING, SEQUENCE AND REGULATION

Citation
Km. Ludin et al., THE ADE4 GENE OF SCHIZOSACCHAROMYCES-POMBE - CLONING, SEQUENCE AND REGULATION, Current genetics, 25(5), 1994, pp. 465-468
Citations number
17
Categorie Soggetti
Genetics & Heredity
Journal title
ISSN journal
01728083
Volume
25
Issue
5
Year of publication
1994
Pages
465 - 468
Database
ISI
SICI code
0172-8083(1994)25:5<465:TAGOS->2.0.ZU;2-U
Abstract
We report the isolation and sequence of the Schizosaccharomyces pombe ade4 gene which encodes the glutamine phosphoribosylpyrophosphate amid otransferase, the first enzyme of the purine nucleotide de-novo biosyn thetic pathway. The enzyme contains 533 amino acids and its sequence e xhibits homologies to the corresponding enzymes of Saccharomyces cerev isiae, Escherichia coli, Bacillus subtilis, chicken, rat, and human. I n contrast to the situation in S. cerevisiae, adenine does not repress ade4 expression at the mRNA level and also other nutritional signals seem not to affect its expression.