DEVELOPMENT OF A SELECTIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR 1-NAPHTHOL - THE MAJOR METABOLITE OF CARBARYL (1-NAPHTHYL N-METHYLCARBAMATE)

selective enzyme-linked immunosorbent assay for 1-naphthol was develop ed. Eight rabbits were immunized with either [(5-hydroxy-2-naphthyleny l)oxy]acetic acid (2b)-BSA, [(5-hydroxy-1-naphthylenyl)oxy]acetic acid (4)-BSA, or 5-hydroxy-2-naphthoic acid (7)-KLH, and all sera were scr eened against 14 different coating antigens. From this screening proce dure, five assays could be developed, using two 7-KLH antisera, 3907 a nd 3909. The assay with the least interference and the best reliabilit y (7-KLH antiserum 3907, 1:9000, and coating antigen 2b-BSA, 1.5 mu g/ mL) was further optimized and characterized. It showed an I-50 value o f about 72 mu g/L, with a range of detection from 10 to 1000 mu g/L. T here was no cross-reactivity with carbaryl, which makes this assay ver y suitable for the selective detection of 1-naphthol, for example, in undiluted human urine and in soil extract. The assay was tested in org anic solvents and showed a tolerance of at least 10% solvent (acetone, acetonitrile, methanol).