CHARACTERIZATION OF NORMOTENSIVE AND HYPE RTENSIVE RAT LINES BY THE DNA-FINGERPRINTING METHOD

The properties of normotensive and hypertensive rat lines were investi gated by the DNA fingerprinting method using a multilocus microsatelli te (CAC)(5) probe. The HaeIII and HinfI restriction endonucleases were found to be the most informative enzymes in this case. The high genet ic homogeneity of the ISIAH line, a rat line with inherited stress-sen sitive arterial hypertension created at the Institute of Cytology and Genetics, was demonstrated. The lack of intralinear polymorphism was a lso typical to Japanese SHR rats with spontaneous arterial hypertensio n. Normotensive WAG rats had identical fingerprinting patterns, while the relative intensity of some bands was different. The outbred normot ensive Wistar line maintained at the Institute, appeared to carry 30% polymorphic alleles. Hypertensive lines differed from the normotensive by a number of genetic markers.