ENDOCYTOSIS AND INTRACELLULAR DEGRADATION OF HETEROLOGOUS PROTEIN BY EOSINOPHILIC GRANULOCYTES ISOLATED FROM RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) POSTERIOR INTESTINE
D. Dorin et al., ENDOCYTOSIS AND INTRACELLULAR DEGRADATION OF HETEROLOGOUS PROTEIN BY EOSINOPHILIC GRANULOCYTES ISOLATED FROM RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) POSTERIOR INTESTINE, Biology of the cell, 79(3), 1993, pp. 219-224
Adherence capacity to tissue substrate, endocytosis capacity for heter
ologous and homologous proteins, and proteolytic activity were determi
ned in intestinal granulocytes (EGCs) isolated from healthy adult rain
bow trout. The percentage of cells that could adhere to a smooth plast
ic surface increased with increasing incubation time. Endocytosis was
effective for heterologous (human immunoglobulin G, IgGh; ovine somato
tropin, oST) but not homologous proteins (recombinant trout somatotrop
in, rtST). The activity of cathepsin D increased significantly after t
he endocytosis of a heterologous protein. Finally, the analysis of imm
unoblots of homogenates of granulocytes incubated in the presence of t
he two different proteins was used to show the endocytosis and degrada
tion of heterologous proteins. These results show that isolated EGCs c
an endocytose and degrade heterologous proteins.