GENETIC-ANALYSIS AND OVEREXPRESSION OF LIPOLYTIC-ACTIVITY IN BACILLUS-SUBTILIS

Authors
DARTOIS V COPPEE JY COLSON C BAULARD A
Citation
V. Dartois et al., GENETIC-ANALYSIS AND OVEREXPRESSION OF LIPOLYTIC-ACTIVITY IN BACILLUS-SUBTILIS, Applied and environmental microbiology, 60(5), 1994, pp. 1670-1673
Citations number
16
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
5
Year of publication
1994
Pages
1670 - 1673
Database
ISI
SICI code
0099-2240(1994)60:5<1670:GAOOLI>2.0.ZU;2-0
Abstract
The previously cloned Bacillus subtilis lipase gene (lip) was mapped o n the chromosome and used in the construction of a B. subtilis derivat ive totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promote r. A 100-fold overproducing strain was obtained, which should facilita te purification of the secreted protein. Furthermore, the Delta lip st rain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.