INHIBITORY EFFECTS OF ENRICHMENT MEDIA ON THE ACCUPROBE TEST FOR LISTERIA-MONOCYTOGENES

Authors
PARTIS L NEWTON K MURBY J WELLS RJ
Citation
L. Partis et al., INHIBITORY EFFECTS OF ENRICHMENT MEDIA ON THE ACCUPROBE TEST FOR LISTERIA-MONOCYTOGENES, Applied and environmental microbiology, 60(5), 1994, pp. 1693-1694
Citations number
4
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
5
Year of publication
1994
Pages
1693 - 1694
Database
ISI
SICI code
0099-2240(1994)60:5<1693:IEOEMO>2.0.ZU;2-P
Abstract
During an evaluation of the Accuprobe kit for the detection of Listeri a monocytogenes, some of the enrichment media used were found to inter fere with the test. Microscopic examination during the lysis step of t he test revealed that media containing high salt greatly reduced or pr evented cell lysis. This prevented the probe from binding to the cellu lar RNA, resulting in false-negative results.