OPTIMIZATION OF THE SISTROM CULTURE-MEDIUM FOR LARGE-SCALE BATCH CULTIVATION OF RHODOSPIRILLUM-RUBRUM UNDER SEMIAEROBIC CONDITIONS WITH MAXIMAL YIELD OF PHOTOSYNTHETIC MEMBRANES
R. Ghosh et al., OPTIMIZATION OF THE SISTROM CULTURE-MEDIUM FOR LARGE-SCALE BATCH CULTIVATION OF RHODOSPIRILLUM-RUBRUM UNDER SEMIAEROBIC CONDITIONS WITH MAXIMAL YIELD OF PHOTOSYNTHETIC MEMBRANES, Applied and environmental microbiology, 60(5), 1994, pp. 1698-1700
The defined medium A of W. R. Sistrom (W. R. Sistrom, J. Gen. Microbio
l. 22:77-85, 1960) has been modified to allow the growth of Rhodospiri
llum rubrum in large-scale batch cultures under dark, semiaerobic cond
itions. The simultaneous use of two substrates, NH4-succinate (46 mM)
and fructose (0.3%), which are utilized in aerobic and fermentative me
tabolism, respectively, leads to very high cell densities with a maxim
al yield of photosynthetic membranes.