STRUCTURE OF THE HUMAN CCG1 GENE - RELATIONSHIP BETWEEN THE EXONS INTRONS AND FUNCTIONAL DOMAIN MODULES OF THE PROTEIN

The human CCG1 gene, encoding CCG1/TAF(II)250/p250, was isolated by co mplementing tsBN462, a mutant BHK21 cell line that shows cell-cycle ar rest at high temperature. Using the cDNA as a probe, the locations of exon-intron junctions were determined in the genomic DNA: Thirty-eight exons ranging from 68 to 219 bp in size were found. All the exon-intr on junctions followed the GT-AG rule. Using a newly developed method, we performed a module analysis of the CCG1 protein. The functional dom ain previously predicted in CCG1 was further confirmed to be encoded i n a single predicted module that is the minimal functional unit in the protein. The boundaries of the predicted modules show a close correla tion to the intron/exon junction of CCG1. The entire gene, at least 11 0 kb long has been recovered in a YAC, which provides a route to the f urther study of module function.