USE OF PROTEINASE-K IN THE EXCYSTATION OF SARCOCYSTIS-CRUZI SPOROCYSTS FOR IN-VITRO CULTURE AND DNA EXTRACTION

Authors
NDIRITU W CAWTHORN RJ KIBENGE FSB
Citation
W. Ndiritu et al., USE OF PROTEINASE-K IN THE EXCYSTATION OF SARCOCYSTIS-CRUZI SPOROCYSTS FOR IN-VITRO CULTURE AND DNA EXTRACTION, Veterinary parasitology, 52(1-2), 1994, pp. 57-60
Citations number
12
Categorie Soggetti
Parasitiology,"Veterinary Sciences
Journal title
Veterinary parasitology → ACNP
ISSN journal
03044017
Volume
52
Issue
1-2
Year of publication
1994
Pages
57 - 60
Database
ISI
SICI code
0304-4017(1994)52:1-2<57:UOPITE>2.0.ZU;2-R
Abstract
Proteinase K was used for the cleaning of Sarcocystis cruzi (Apicomple xa) sporocysts prior to excystation. Bovine pulmonary endothelial cell cultures inoculated with the excysted sporozoites remained free of ba cterial contamination for the duration of the experiment and had high yields of merozoites. The excysted sporozoites also yielded genomic DN A that could be labelled efficiently with P-32 dATP by the random prim ing method.