TOWARDS HIGH-RESOLUTION MAPS OF THE MOUSE AND HUMAN GENOMES - A FACILITY FOR ORDERING MARKERS TO 0.1 CM RESOLUTION

982 progeny produced by a mouse interspecific backcross between C57BL/ 6 and Mus spretus have been scored for at least 3 markers on each chro mosome, completing an anchor map of 78 loci across the mouse genome. T he anchor mapping identifies all the available recombinants in each in teranchor interval allowing access to panels of mice that can be used for the high resolution mapping of any chromosome region. The large nu mber of progeny recovered and scored from the interspecific backcross allows us to resolve genetically markers that lie on average 200 kb ap art on mouse chromosomes and within the cloning capacity of currently available YAC libraries. EUCIB provides the first genetic mapping reso urce specifically designed for the high resolution mapping of all regi ons of the mouse genome and will underpin the global physical mapping of the mouse genome. In addition, with the use of conserved sequences the facility is applicable to the high resolution comparative mapping of the mouse and human genomes. A new database has been implemented to support the computation of high resolution and ordered genetic maps.