COLUMN CHROMATOGRAPHY AND KINETICS OF NUCLEOSIDES AND NUCLEIC-ACID BASES ON IMMOBILIZED NICKEL-CDAE-SPOROPOLLENIN AND COBALT-CDAE-SPOROPOLLENIN

Sporopollenin, a natural polymer, has been modified for application as a ligand-exchange material. The ligand-exchange chromatography with t he functionalized Lycopodium clavatum is a useful method for the rapid separation of nucleosides and nucleic acid bases. The synthesis of tr is(carboxymethyl) ethylene diamine Lycopodium clavatum has been descri bed. The resin contained functional diaminoethane and carboxyl groups. Nickel(II) and cobalt(II) metal ions can easily be immobilized on thi s carboxylated-diamino-ethyl (CDAE)-sporopollenin. Physicochemical and chelating properties of Lycopodium clavatum have been studied extensi vely. Ligand-exchange chromatography of CDAE-sporopollenin has also be en compared to the conventional synthetic chelex-100 resin. The kineti cs of cytidine in CDAE-sporopollenin resin has been investigated. The rate measurements have been made by a potentiometric technique. The re lative rates at which nucleosides bind to the resin are determined by the actual chemical-exchange reaction between ligands and resin.