CHIRAL HPLC WITH CARBOHYDRATE CARBAMATES - INFLUENCE OF SUPPORT STRUCTURE ON ENANTIOSELECTIVITY

The effect of particle size and pore size of the aminopropylated silic a support for cellulose tris(phenylcarbamate) and tris(3,5-dimethylphe nylcarbamate) chiral HPLC phases was investigated. It was necessary to reduce phase loading below 20% w/w as pore size and particle size wer e reduced, but high efficiency columns could be prepared at a 15% w/w loading on 5 and 2.5 mu m supports with 120-Angstrom-diameter pores. T he 2.5 mu m phase permits the use of relatively high flow rates and ve ry efficient enantioselective separations of a range of chiral compoun ds could be achieved in less than 3 min. (C) 1994 Wiley-Liss, Inc.