Heat treatment, protein concentration, and pH effects on water vapor p
ermeability (WVP) of plasticized whey protein films were examined. The
best film formation conditions were neutral pH, aqueous 10% (w/w) pro
tein solutions heated for 30 min at 90 degrees C. Isoelectric point ad
justment of whey protein with calcium ascorbate buffer increased WVP w
ith increasing buffer concentration. The importance of vacuum applicat
ion to minimize film pore size was identified using scanning electron
microscopy. Polyethylene glycol, glycerol and sorbitol plasticizer con
centration affected film WW. Determining the effects of relative humid
ity on WVP for plasticized whey protein films enabled prediction of fi
lm behavior under any water vapor partial pressure gradient.