A NOVEL EX-VIVO METHOD FOR ASSAYING ADHESION OF CANCER-CELLS TO THE PERITONEUM

A novel ex vivo method to determine the cell adhesion of cancer cells to the peritoneum was described. The wells of a microtiter plate were filled with cell suspension and sealed using mouse peritoneum. The per itoneum was fixed using a plastic cover and the plate was turned upsid e down and incubated for cell adhesion. After incubation for 80 min, t he plate was centrifuged and non-adherent cells were assayed by MTT as say. Human cancer cells (MKN28, MKN45, MKN74, KM12C and KM12SM) adhere d to the mouse peritoneum as well as cells from mouse (Colon26) and th e ratio of cells attached to the peritoneum was estimated to be betwee n 12.8 and 66.4%. This simple method could be useful to investigate th e adhesion molecule associated with peritoneal dissemination.