PREPARATION AND CHARACTERIZATION OF A S-MONOMETHOXYPOLY-(ETHYLENE GLYCOL) THIODERIVATIVE OF PAPAIN

Authors
PAUL C VINCENTELLI J BRYGIER J MUSU T BAEYENSVOLANT D GUERMANT C LOOZE Y
Citation
C. Paul et al., PREPARATION AND CHARACTERIZATION OF A S-MONOMETHOXYPOLY-(ETHYLENE GLYCOL) THIODERIVATIVE OF PAPAIN, Phytochemistry, 35(6), 1994, pp. 1413-1417
Citations number
31
Categorie Soggetti
Plant Sciences
Journal title
Phytochemistry → ACNP
ISSN journal
00319422
Volume
35
Issue
6
Year of publication
1994
Pages
1413 - 1417
Database
ISI
SICI code
0031-9422(1994)35:6<1413:PACOAS>2.0.ZU;2-W
Abstract
A derivative of papain in which the free thiol group of the proteinase is attached to a poly(ethylene glycol) chain via a disulphide bond ha s been prepared. The poly(ethylene glycol) chain confers to the conjug ate new physicochemical properties, including a looser binding to ion exchange chromatographic supports such as CM-Sephadex C-50. The conjug ate is thus readily separated from the irreversibly oxidized (unconjug ated) forms of the enzyme. A papain preparation titrating 0.97 mol of thiol per mol of enzyme is obtained from the conjugate.