S. Yamasaki et al., BOTULINUM NEUROTOXIN TYPE-G PROTEOLYSES THE ALA(81)-ALA(82) BOND OF RAT SYNAPTOBREVIN-2, Biochemical and biophysical research communications, 200(2), 1994, pp. 829-835
Tetanus toxin and the botulinum neurotoxins types A to F inhibit neuro
transmitter release from presynaptic nerve endings by selectively prot
eolysing the synaptic proteins synaptobrevin, syntaxin, or SNAP-25. He
re, we show that botulinum toxin type G cleaves rat synaptobrevin 2 be
tween Ala(81) and Ala(82), a peptide bond that differs from those atta
cked by tetanus toxin and the botulinal toxins types B, D, and F. Syna
ptobrevin isoforms carrying a Gly in the P1 position are poor substrat
es. Analyses of N-terminal deletion mutants of rat synaptobrevin 2 sho
wed that a substrate starting at Leu(54) is cleaved efficiently, where
as substrates beginning at Leu(60) or Phe(77) are cleaved partially or
not at all, respectively. (C) 1994 Academic Press, Inc.