M. Romaris et al., RAS TRANSFORMATION ALTERS THE COMPOSITION OF EXTRACELLULAR-MATRIX PROTEOGLYCANS IN RAT FIBROBLASTS, Biochemical and biophysical research communications, 200(2), 1994, pp. 925-932
ts371 KiMuSV-transformed rat kidney fibroblasts growing at the permiss
ive temperature to allow the expression of a transforming p21 ras prot
ein secrete a different pattern of chondroitin/dermatan sulfate proteo
glycans than when growing at the non-permissive temperature. Transform
ed cells produce less versican and larger amounts of biglycan and deco
rin than their normal counterpart, and they do not secrete S-35-labele
d PG-100. Western blot experiments showed the presence of PG-100 epito
pe, suggesting that the apparent effect of the oncoprotein is due to a
reduced sulfation of the molecule. Transformed and non-transformed ce
lls are similarly sensitive to the proteoglycan-induction effect of TG
F-beta. (C) 1994 Academic Press, Inc.