A. Tsuji et al., IDENTIFICATION OF NOVEL CDNAS ENCODING HUMAN KEXIN-LIKE PROTEASE, PACE4 ISOFORMS, Biochemical and biophysical research communications, 200(2), 1994, pp. 943-950
PACE4 has been identified as a second human subtilisin-like protease b
y Keifer et al. [DNA and Cell Biology (1991) 10, 757-769]. In this stu
dy, we isolated two novel cDNAs coding for PACE4 isoforms (PACE4C and
PACE4D) from a human placenta cDNA library. The deduced PACE4C protein
sequence (652 amino acids) lacks the cysteine-rich region located at
the carboxy terminus of PACE4. The DNA sequence of the 3'-untranslated
region (1 kb) of PACE4C is not homologous to the PACE4 sequence. The
protein (497 amino acids) encoded by PACE4D cDNA lacks a signal peptid
e, a propeptide and the cysteine-rich region. These isoform cDNAs were
also isolated from rat pituitary cDNA library. (C) 1994 Academic Pres
s, Inc.