Md. Castillo et al., DETERMINATION OF MANGANESE PEROXIDASE-ACTIVITY WITH 3-METHYL-2-BENZOTHIAZOLINONE HYDRAZONE AND 3-(DIMETHYLAMINO)BENZOIC ACID, Analytical biochemistry, 218(2), 1994, pp. 399-404
This method was proposed earlier for measuring glucose in a peroxidase
-glucose oxidase system but has not been studied for determination of
manganese peroxidase (MnP) activity. The assay is based on the oxidati
ve coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 3-(di
methylamino)benzoic acid (DMAB). The reaction of MBTH and DMAB in the
presence of H2O2, Mn2+, and MnP gives a deep purple-blue color with a
broad absorption band with a peak at 590 nm. The extinction coefficien
t is high (53,000 M(-1) cm(-1)), so low MnP activities can be detected
. Lignin peroxidase and laccase, usually present in cultures of white
rot fungi, gave little or no interference at the concentrations tested
. However, slight interference from very high LiP activity may occur a
t very low MnP activity. (C) 1994 Academic Press, Inc.