CONFIRMATION OF IMMOBILIZING IGG ON DIFFERENT SURFACES WITH AFM AND IMPEDANCE INVESTIGATIONS OF A PT ELECTRODE DURING THE IMMOBILIZATION

Immobilization of anti-SEB on SiO2, Pt and Au thin films have been car ried out by using a conventional covalent bonding technique. AFM inves tigation showed that after the immobilization, a monolayer of the anti body molecules was formed on the SiO2 film, By reacting the immobilize d anti-SEB with gold colloid conjugated anti-IgG, gold particles were observed on the antibody immobilized film surface by AFM. Pt films sho wed the highest coverage of anti-SEB after immobilization. Impedance c hange of a Pt electrode in PBS solution was also investigated before a nd after the immobilization of anti-SEB.